WRIGHT-GIEMSA Stain
Buffered HANSEL® Modified
One Minute Technic
FOR IN VITRO DIAGNOSTIC USE
For blood smears, exfoliative and exudative cytology
Our WRIGHT-GIEMSA Stain contains a combination of dyes in a buffered methanol solution with a similar glycerin-water base formula as HANSEL Stain. The product incorporates the resolution of cellular details obtained from Giemsa Stain and additional Eosin-Y Stain with the rapid staining time of Wright Stain. Slides can be read with a minimum of preparation time. Results are excellent in blood, bone marrow preparations and inflammatory exudates of all types.

DIRECTIONS
1. Make thin blood smears on clear slides.
2. For exfoliative and exudative cytology, fix smears immediately by dipping several times in alcohol-ether (equal parts) or store in same solution.
3. Flood slide with Stain, wait about 30 seconds.
4. Add distilled water. Blow gently to mix.
5. Pour off after 30 seconds. Wash immediately with distilled water.
6. Dry by waving in air.

CYTOCHEMISTRY
With its combination of acidic and basis dyes, Lide Laboratories WRIGHT-GIEMSA Stain can differentiate granules, cytoplasm and nuclei of various cell types. The buffered solvent and base formula produce an effective vehicle to evenly carry the stain. Cytochemical staining produces a predictable color pattern that can microscopically distinguish cells and cell morphology. Referenced differential staining morphology of blood cell types include:

Red blood cells: cytoplasm = orange to pint to rose
Lymphocytes: cytoplasm = light blue / nucleus = deep blue violet
Eosinophils: granules = red to red-orange
Platelets: central granules = red-purple surrounded by light blue
Monocytes: cytoplasm = pale gray-blue / nucleus = deep bluish purple
Neutrophils:granules = purple to lilac / cytoplasm = pale pink / nucleus = deep blue violet
Basophils: granules = deep blue to violet

Color and color distribution may vary due to the stain, specimen type, and the conditions of the testing including specimen collection, sample preparation and fixation, stain application and rinse.

PRODUCT DATA
CONTENTS: Buffered Methanol, Glycerol, Wright Stain, Giemsa Stain, Eosin-Y Stain

FLAMMABLE. Avoid exposure to excessive heat or flame.

Do not ingest. Avoid contact with skin, hands and eyes.

Material Safety Data Sheet
May be used to comply with
OSHA's Hazard Communication Standard,
29 CFR 1910.1200. Standard must be
consulted for specific requirements.
U.S. Department of Labor
Occupational Safety and Health Administration (Non-Mandatory Form) Form Approved OMB No. 1218-0072

IDENTITY (As Used on Label and List)
WRIGHT-GEIMSA STAIN and WRIGHT-GIEMSA V-Cell Stain

Section I
Manufacturer's Name:
Lide Laboratories Inc. Emergency Telephone Number: 952-758-9760 or contact a local medical facility
Address (Number, Street, City, State, and ZIP Code) Telephone Number for Information: 952-758-9760
401 4th AVE SW Date Prepared: 01/17/2011
New Prague, MN 56071 Signature of Preparer (optional)
The statements contained are offered as information only and are believed to be accurate and represent the best information currently available. However, we make no warranty, expressed or implied, with respect to such information and assume no liability or any type resulting form its use. Users of the product should make their own investigations to determine suitability
of the information for their particular purposes.
Section II - Hazardous Ingredients/Identity Information
Hazardous Components (Specific Chemical Identity; Common Name(s)) OSHA PEL ACGIH TLV Other Limits Recommended %(optional)
95% Buffered Methanol (Synonyms: Carbinol; Methyl alcohol; Methyl hydroxide; Monohydroxymethane; Wood alcohol; Wood naptha; Wood spirits; Columbian spirits) CAS RN: 67-56-1 PEL/TLV/TWA: 200 ppm
Buffered with Potassium and Sodium Phosphate Monobasic and Dibasic
Other Ingredients 5%: Wright Stain, Giemsa Stain, Eosin Y Stain, Glycerin, Purified Water
EMERGENCY OVERVIEW
Danger! Flammable liquid and vapor.
Poison! Methanol may be fatal or cause blindness if swallowed. Vapor harmful. Harmful if swallowed, inhaled, or absorbed through the skin. Causes eye, skin, and respiratory tract irritation. May cause central nervous system depression. Cannot be made non-poisonous.
Section III - Physical/Chemical Characteristics
Boiling Point 64.5 ° C Specific Gravity (H2O = 1) 0.79
Vapor Pressure (mm Hg) 96 mm Hg @ 20 ° C Melting Point -98° C
Vapor Density (AIR = 1) 1.1 Evaporation Rate (Butyl Acetate = 1) 5.91
Solubility in Water: Complete FLASHPOINT 12.2 C
Appearance and Odor: Blue in color as WRIGHT-GIEMSA Stain with slight alcoholic odor.
Section IV - Fire and Explosion Hazard Data
Flash Point (Method Used) 12.2 ° C Flammable Limits LEL 6.7 UEL 35
Extinguishing Media: For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. Water may be ineffective. For large fires, use water spray, fog or alcohol-resistant foam. Do NOT use straight streams of water.
Special Fire Fighting Procedures: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed containers cool. Water may be ineffective. Material is lighter than water and a fire may be spread by the use of water. Extinguish all nearby sources of ignition, Vapors are heavier than air and may travel to a source of ignition and flash back. Vapors can spread along the ground and collect in low or confined areas
Unusual Fire and Explosion Hazards: Methanol may burn with a flame that is invisible in the daylight. Mixtures of water and as little as 21% methanol are flammable. This includes these Stains.
Section V - Reactivity Data
Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: High temperatures, ignition sources, confined spaces.
Incompatibilities with Other Materials: Oxidizing agents, reducing agents, acids, alkali metals, potassium, sodium, metals as powders (e.g. hafnium, raney nickel), acid anhydrides, acid chlorides, powdered aluminum, powdered magnesium.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, formaldehyde.
Hazardous Polymerization: Will not occur.
Section VI - Health Hazard Data
Health Hazards (Acute and Chronic)
Danger! Flammable liquid and vapor.
Poison! Methanol may be fatal or cause blindness if swallowed. Vapor harmful. Harmful if swallowed, inhaled, or absorbed through the skin. Causes eye, skin, and respiratory tract irritation. May cause central nervous system depression. Cannot be made non-poisonous.
Emergency and First Aid Procedures
Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes.
Get immediate medical attention. WRIGHT-GIEMSA Stains will stain eyes.

Ingestion: Potential for aspiration if swallowed. Get medical aid immediately. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If vomiting occurs naturally, have victim lean forward.
Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Skin: In case of contact, immediately wash skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. WRIGHT=GIEMSA Stains will stain skin. Get medical attention if irritation persists after washing.
Notes to Physician: Effects may be delayed.
Antidote: Ethanol may inhibit methanol metabolism.
Potential Health Effects as methanol:
Eye: May cause painful sensitization to light. Methanol is a mild to moderate eye irritant. Inhalation, ingestion or skin absorption of methanol can cause significant disturbances in vision, including blindness.
Skin: Causes moderate skin irritation. May be absorbed through the skin in harmful amounts. Prolonged and/or repeated contact may cause defatting of the skin and dermatitis. Methanol can be absorbed through the skin, producing systemic effects that include visual disturbances.
Ingestion: May be fatal or cause blindness if swallowed. Aspiration hazard. Cannot be made non-poisonous. May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause systemic toxicity with acidosis. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. May cause cardiopulmonary system effects.
Inhalation: Methanol is toxic and can very readily form extremely high vapor concentrations at room temperature. Inhalation is the most common route of occupational exposure. At first, methanol causes CNS depression with nausea, headache, vomiting, dizziness and in coordination. A time period with no obvious symptoms follows (typically 8-24 hrs). This latent period is followed by metabolic acidosis and severe visual effects which may include reduced reactivity and/or increased sensitivity to light, blurred, double and/or snowy vision, and blindness. Depending on the severity of exposure and the promptness of treatment, survivors may recover completely or may have permanent blindness, vision disturbances and/or nervous system effects.
Chronic: Prolonged or repeated skin contact may cause dermatitis. Chronic exposure may cause effects similar to those of acute exposure. Methanol is only very slowly eliminated from the body. Because of this slow elimination, methanol should be regarded as a cumulative poison. Though a single exposure may cause no effect, daily exposures may result in the accumulation of a harmful amount. Methanol has produced fetotoxicity in rats and teratogenicity in mice exposed by inhalation to high concentrations that did not produce significant maternal toxicity.
Section VII - Precautions for Safe Handling and Use
Steps to Be Taken in Case Material is Released or Spilled: Use proper personal protective equipment as indicated in Section VIII. Avoid direct contact with the product. WRIGHT-GEIMSA Stain will stain the skin. Lava Soap has been found to be a good cleansing agent. Absorb spill using an absorbent, non-combustible material such as earth, sand, or vermiculite. Do not use combustible materials such as sawdust. Use a spark-proof tool. Provide ventilation. A vapor suppressing foam may be used to reduce vapors. Water spray may reduce vapor but may not prevent ignition in closed spaces.
Waste Disposal Method: Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. RCRA P-Series: None listed. RCRA U-Series: Methanol CAS# 67-56-1: waste number U154 (Ignitable waste).
Precautions to Be taken in Handling and Storing
Handling: Avoid contact with eyes, skin, and clothing. Do not ingest or inhale. Use only with adequate ventilation. Keep away from heat, sparks and flame. Avoid use in confined spaces.
Follow good laboratory practices and product use instructions.
Storage: Keep container tightly closed. Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store in a cool, dry, well-ventilated area away from incompatible substances. Protect from light.

Transport Information For Containers over 1 oz.
Shipping Name: METHANOL SOLUTION, Hazard Class: 3, UN Number: UN1230, Packing Group: II

CHEMICAL STORAGE CODES: HEALTH 3 FLAMMABILIITY 3 REACTIVITY 0 PERSONAL PROTECTION 1
Section VIII - Control Measures
Engineering Controls:. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits. A fume hood is recommended.
OSHA Vacated PELs: Methanol: 200 ppm TWA; 260 mg/m3 TWA
Personal Protective Equipment
Eyes: Wear chemical splash goggles. A face shield may be necessary.
Skin: Wear butyl rubber gloves, apron, and/or clothing.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.